New York Attorney General Letitia James has accused Valve of promoting illegal gambling through its video games in a lawsuit filed by her office. According to the AG’s announcement, her office conducted an investigation and had concluded that Valve enabled gambling by enticing users to pay for a chance at rare items from loot boxes in Counter-Strike 2, Team Fortress 2 and Dota 2. In the lawsuit, the New York AG stressed that Valve’s loot boxes are “particularly pernicious,” because the games are popular among children and teenagers.
This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
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